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Proceedings Paper

The spectroscopic manifestation of the interaction of some cyanine dyes with BSA and DNA
Author(s): Eldar N. Kurtaliev; Negmat Nizomov; Sherzod I. Rahimov; Gayrat Khodjayev; Dilorom P. Khakimova
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Paper Abstract

The spectral-luminescent characteristics of cyanine dye Cyan 40 and thiazole orange at interaction with biomacromolecules bovine serum albumin and deoxyribonucleic acid was studied. It is shown that presence of biomacromolecules in solution of the studied species leads to the change of spectral-luminescent characteristics of the dyes Cyan 40 and TO. The observed phenomena in absorption spectrum and fluorescence of the studied dyes is explained by complex formation between dye and biological macromolecules. The binding parameters: a binding constant (K) and quantity of the binding sites (N) the studied dyes with biomacromolecules are determined.

Paper Details

Date Published: 26 February 2010
PDF: 5 pages
Proc. SPIE 7571, Single Molecule Spectroscopy and Imaging III, 757116 (26 February 2010); doi: 10.1117/12.840722
Show Author Affiliations
Eldar N. Kurtaliev, Samarkand State Univ. (Uzbekistan)
Negmat Nizomov, Samarkand State Univ. (Uzbekistan)
Sherzod I. Rahimov, Samarkand State Univ. (Uzbekistan)
Gayrat Khodjayev, Samarkand Agricultural Institute (Uzbekistan)
Dilorom P. Khakimova, Consultative and Diagnostic Hospital of Samarkand (Uzbekistan)


Published in SPIE Proceedings Vol. 7571:
Single Molecule Spectroscopy and Imaging III
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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