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Proceedings Paper

Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation
Author(s): Bevin Lin; Shiro Urayama; Ramez M. G. Saroufeem; Dennis L. Matthews; Stavros G. Demos
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Paper Abstract

Epithelial microstructures related to early stage carcinoma are currently invisible to traditional white light endoscopy. We recently demonstrated that autofluorescence microscopy under ultraviolet excitation can visualize superficial microstructures without the use of contrast agents, sectioning methods, or tissue preparation. Spectroscopic analysis allowed a better understanding of autofluorescence signal characteristics at the microscopic level and the mechanism for achieving high quality imaging of the superficial epithelial layer with conventional wide-field microscopy. The designing parameters for the adaptation of this technology into an endoscope probe for real-time in vivo microscopy are tested using a bench-top prototype system. This approach may provide a powerful tool for the detection and staging of carcinomas.

Paper Details

Date Published: 11 February 2010
PDF: 8 pages
Proc. SPIE 7561, Optical Biopsy VII, 756103 (11 February 2010); doi: 10.1117/12.840715
Show Author Affiliations
Bevin Lin, Univ. of California, Davis (United States)
Shiro Urayama, Univ. of California, Davis Medical Ctr. (United States)
Ramez M. G. Saroufeem, Univ. of California, Davis Medical Ctr. (United States)
Dennis L. Matthews, Univ. of California, Davis (United States)
Stavros G. Demos, Univ. of California, Davis (United States)
Lawrence Livermore National Lab. (United States)


Published in SPIE Proceedings Vol. 7561:
Optical Biopsy VII
Robert R. Alfano, Editor(s)

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