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Proceedings Paper

Photoacoustic microscopy using Evans Blue dye as a contrast agent
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Paper Abstract

Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBC) as an endogenous contrast agent. However, intermittent RBC flow in capillaries results in discontinuous and fragmentary capillary images. To overcome this problem, we used Evans Blue (EB) dye as a contrast agent for in vivo photoacoustic imaging. EB has strong optical absorption at 610 nm and distributes uniformly in the blood stream by chemically binding to albumin. By intravenous injection of EB (6%, 200 μL), complete and continuous microvascular networks-especially capillaries-of the ears of nude mice were imaged. The diffusion of EB (3%, 100 μL) leaving the blood stream was monitored for 2 hours. At lower administration dose of EB (3%, 50 μL), the clearance of the EB-albumin complex was imaged for 10 days and quantitatively investigated using a two-compartment model.

Paper Details

Date Published: 23 February 2010
PDF: 6 pages
Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75642J (23 February 2010); doi: 10.1117/12.840684
Show Author Affiliations
Junjie Yao, Washington Univ. in St. Louis (United States)
Konstantin I. Maslov, Washington Univ. in St. Louis (United States)
Song Hu, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 7564:
Photons Plus Ultrasound: Imaging and Sensing 2010
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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