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Proceedings Paper

Quantitative analysis of thermally-induced alterations of corneal stroma by second-harmonic generation imaging
Author(s): P. Matteini; F. Rossi; F. Ratto; R. Cicchi; D. Kapsokalyvas; F. S. Pavone; R. Pini
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Paper Abstract

Thermal modifications induced in the corneal stroma were investigated by means of second harmonic generation (SHG) imaging. Whole fresh cornea samples were heated in a water bath at temperatures in the 35-80 °C range for a 4-min time. SHG images of the structural modifications induced at each temperature were acquired from different areas of cross-sectioned corneal stroma by using an 880 nm linearly- and circularly-polarized excitation light emitted by a mode-locked Ti:Sapphire laser. The SHG images were then analyzed by means of both an empirical approach and a 2D-theoretical model. The proposed analyses provide a detailed description of the changes occurring in the structural architecture of the cornea during the thermal treatment. Our results allow us to depict a temperature-dependent biochemical model for the progressive destructuration occurring to collagen fibrils and nonfibrillar components of the stroma.

Paper Details

Date Published: 2 March 2010
PDF: 5 pages
Proc. SPIE 7550, Ophthalmic Technologies XX, 755022 (2 March 2010); doi: 10.1117/12.840674
Show Author Affiliations
P. Matteini, Istituto di Fisica Applicata Nello Carrara, CNR (Italy)
F. Rossi, Istituto di Fisica Applicata Nello Carrara, CNR (Italy)
F. Ratto, Istituto di Fisica Applicata Nello Carrara, CNR (Italy)
R. Cicchi, Univ. degli Studi di Firenze (Italy)
D. Kapsokalyvas, Univ. degli Studi di Firenze (Italy)
F. S. Pavone, Univ. degli Studi di Firenze (Italy)
R. Pini, Istituto di Fisica Applicata Nello Carrara, CNR (Italy)


Published in SPIE Proceedings Vol. 7550:
Ophthalmic Technologies XX
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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