Share Email Print
cover

Proceedings Paper

Non-negative matrix factorization: a blind sources separation method applied to optical fluorescence spectroscopy and multiplexing
Author(s): Anne-Sophie Montcuquet; Lionel Hervé; Jean-Marc Dinten; Jérôme I. Mars
Format Member Price Non-Member Price
PDF $17.00 $21.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Fluorescence optical imaging use one or several (in multiplexing) injected fluorescent markers which specifically bind to targeted compounds. Near infrared light illuminates the region of interest and the emitted fluorescence is analyzed to localize fluorescence sources. A spectroscopic approach and a separation source method (Nonnegative matrix factorization) are explored to separate different fluorescence sources and remove the unwanted biological tissues autofluorescence. We present unmixing results on overlapping spectra of interest, and show that autofluorescence removal improves Fluorescent Diffuse Optical Tomography.

Paper Details

Date Published: 26 February 2010
PDF: 5 pages
Proc. SPIE 7573, Biomedical Applications of Light Scattering IV, 757314 (26 February 2010); doi: 10.1117/12.840648
Show Author Affiliations
Anne-Sophie Montcuquet, Lab. d'Electronique de Technologie de l'Information, CEA (France)
GIPSA-Lab., DIS (France)
Lionel Hervé, Lab. d'Electronique de Technologie de l'Information, CEA (France)
Jean-Marc Dinten, Lab. d'Electronique de Technologie de l'Information, CEA (France)
Jérôme I. Mars, GIPSA-Lab., DIS (France)


Published in SPIE Proceedings Vol. 7573:
Biomedical Applications of Light Scattering IV
Adam P. Wax; Vadim Backman, Editor(s)

© SPIE. Terms of Use
Back to Top