Share Email Print
cover

Proceedings Paper

Magnetomotive optical coherence elastography for relating lung structure and function in cystic fibrosis
Author(s): Raghav Kumar Chhetri; Jerome Carpenter; Richard Superfine; Scott H. Randell; Amy Lynn Oldenburg
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Cystic fibrosis (CF) is a genetic defect in the cystic fibrosis transmembrane conductance regulator protein and is the most common life-limiting genetic condition affecting the Caucasian population. It is an autosomal recessive, monogenic inherited disorder characterized by failure of airway host defense against bacterial infection, which results in bronchiectasis, the breakdown of airway wall extracellular matrix (ECM). In this study, we show that the in vitro models consisting of human tracheo-bronchial-epithelial (hBE) cells grown on porous supports with embedded magnetic nanoparticles (MNPs) at an air-liquid interface are suitable for long term, non-invasive assessment of ECM remodeling using magnetomotive optical coherence elastography (MMOCE). The morphology of ex vivo CF and normal lung tissues using OCT and correlative study with histology is also examined. We also demonstrate a quantitative measure of normal and CF airway elasticity using MMOCE. The improved understanding of pathologic changes in CF lung structure and function and the novel method of longitudinal in vitro ECM assessment demonstrated in this study may lead to new in vivo imaging and elastography methods to monitor disease progression and treatment in cystic fibrosis.

Paper Details

Date Published: 19 February 2010
PDF: 10 pages
Proc. SPIE 7554, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV, 755420 (19 February 2010); doi: 10.1117/12.840592
Show Author Affiliations
Raghav Kumar Chhetri, The Univ. of North Carolina at Chapel Hill (United States)
Jerome Carpenter, The Univ. of North Carolina at Chapel Hill (United States)
Richard Superfine, The Univ. of North Carolina at Chapel Hill (United States)
Scott H. Randell, The Univ. of North Carolina at Chapel Hill (United States)
Amy Lynn Oldenburg, The Univ. of North Carolina at Chapel Hill (United States)


Published in SPIE Proceedings Vol. 7554:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV
Joseph A. Izatt; James G. Fujimoto; Valery V. Tuchin, Editor(s)

© SPIE. Terms of Use
Back to Top