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Proceedings Paper

Off-axis phase-shifting holographic interferometry for the 3D localization of cellular transmembrane receptors tagged with gold nanomarkers
Author(s): Fadwa Joud; Nilanthi Warnasooriya; Philippe Bun; Gilles Tessier; Maïte Coppey-Moisan; Michael Atlan; Pierre Desbiolles; Marie Abboud; Michel Gross
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Paper Abstract

In this communication, we show that off-axis digital holography combined to phase-shifting acquisition of holograms is an effective microscopic tool to fully localize, in three dimensions, transmembrane receptors of living cells tagged with Gold nanocolloids. Gold nanoparticles, known for their interesting optical properties as well as for their noncytotoxicity are used here as biomarkers to target the cellular receptors.

Paper Details

Date Published: 17 February 2010
PDF: 4 pages
Proc. SPIE 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II, 757610 (17 February 2010); doi: 10.1117/12.840496
Show Author Affiliations
Fadwa Joud, Lab. Kastler Brossel, École Normale Supérieure (France)
Univ. Saint-Joseph (Lebanon)
Nilanthi Warnasooriya, Institut Langevin, École Supérieure de Physique et de Chimie Industrielles (France)
Philippe Bun, Institut Jacques Monod (France)
Gilles Tessier, Institut Langevin, École Supérieure de Physique et de Chimie Industrielles (France)
Maïte Coppey-Moisan, Institut Jacques Monod (France)
Michael Atlan, Institut Langevin, École Supérieure de Physique et de Chimie Industrielles (France)
Pierre Desbiolles, Lab. Kastler Brossel, École Normale Supérieure (France)
Marie Abboud, Univ. Saint-Joseph (Lebanon)
Michel Gross, Lab. Kastler Brossel, École Normale Supérieure (France)


Published in SPIE Proceedings Vol. 7576:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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