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Proceedings Paper

Controllable infrared continuum source for multiphoton imaging
Author(s): C. de Mauro; D. Alfieri; M. Arrigoni; D. Armstrong; F. S. Pavone
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Paper Abstract

We report on multiphoton imaging of biological samples performed with continuum infrared source generated in photonic crystal fibers (PCFs). We studied the spectra generated in PCFs with dispersion profiles designed to maximize the power density in the 700-1000 nm region, where the two-photon absorption cross sections of the most common dyes lie. Pumping in normal dispersion region, with <140 femtosecond pulses delivered by a tunable Ti:Sa laser (Chameleon Ultra II by Coherent Inc.), results in a limitation of nonlinear broadening up to a mean power density above 2 mW/nm. Axial and lateral resolution obtained with a scanning multiphoton system has been measureed to be near the theoretical limit. The possibility of simultaneous two-photon excitation of different dyes in the same sample and high image resolution are demonstrated at tens of microns in depth. Signal-to-noise ratio and general performances are found to be comparable with those of a single wavelength system, used for comparison.

Paper Details

Date Published: 26 February 2010
PDF: 6 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756919 (26 February 2010); doi: 10.1117/12.840476
Show Author Affiliations
C. de Mauro, Light4Tech Firenze S.r.l. (Italy)
D. Alfieri, Light4Tech Firenze S.r.l. (Italy)
M. Arrigoni, Coherent, Inc. (United States)
D. Armstrong, Coherent, Inc. (United States)
F. S. Pavone, Univ. of Florence (Italy)

Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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