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Proceedings Paper

DNA biosensors based on layer-by-layer self-assembled multilayer films of carbon nanotubes and gold nanoparticles
Author(s): Yiyun Xiao; Zhao Dai; Jimei Zhang; Jiechun Pang; Shichao Xu; Guo Zheng
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Paper Abstract

A novel DNA biosensor based on layer-by-layer self-assembled multi-walled carbon nanotubes (MWNTs) and gold nano-particles (GNPs) was presented in this paper, in which the probe HS-ssDNA oligonucleotides, MWNTs and GNPs were all covalently immobilized by chemical Au-Sulphide bonding. Firstly, the super short MWNTs were prepared and modified with thio groups which could be self-assembled onto the surface of Au elcetrode by Au-sulphide bonding, then the GNPs were chemically adhered to the surfaces of MWNTs by forming Au-sulphide bonding again, at last the selfassamble of probe DNA oligonucleotides were also covalently immobilized via Au-sulphide bonding between thio groups at the ends of the DNA oligonucleotides and GNPs. Hybridization between the probe HS-ssDNA oligonucleotides and target DNA oligonucleotides was confirmed by the changes in the voltammetric peak of an anionic intercalator, anthraquinone-2,6-disulfonic acid (AQDS) as a hybridization indicator. The cyclic voltammetric and differential pulse voltammetry responses demonstrated that the DNA biosensors based on Layer-by-layer self-assembled multilayer films of MWNTs and NGPs offer a higher hybridization efficiency and selectivity compared to those based on only random MWNTs or GNPs.

Paper Details

Date Published: 20 October 2009
PDF: 5 pages
Proc. SPIE 7493, Second International Conference on Smart Materials and Nanotechnology in Engineering, 74935A (20 October 2009); doi: 10.1117/12.840316
Show Author Affiliations
Yiyun Xiao, Tianjin Polytechnic Univ. (China)
Zhao Dai, Tianjin Polytechnic Univ. (China)
Jimei Zhang, Tianjin Polytechnic Univ. (China)
Jiechun Pang, Tianjin Polytechnic Univ. (China)
Shichao Xu, Tianjin Polytechnic Univ. (China)
Guo Zheng, Tianjin Polytechnic Univ. (China)

Published in SPIE Proceedings Vol. 7493:
Second International Conference on Smart Materials and Nanotechnology in Engineering
Jinsong Leng; Anand K. Asundi; Wolfgang Ecke, Editor(s)

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