Share Email Print
cover

Proceedings Paper

High-efficiency single molecule fluorescence detection and correlation spectroscopy with dielectric microspheres
Author(s): Heykel Aouani; Nadia Djaker; Jérôme Wenger; Hervé Rigneault
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Microspheres made of high refractive index melamine resin are shown to enhance the fluorescence from single molecules in solution by seven-fold, and simultaneously reduce the observation volume by thirteen-fold, as compared to state-of-the-art confocal microscopy. This fluorescence enhancement is demonstrated to dramatically increase the signal-to-noise ratio in fluorescence correlation spectroscopy and reduce the experiment integration time by fifty-fold. We also provide the first description of dual-color fluorescence cross-correlation spectroscopy (FCCS) enhanced by a dielectric microsphere, and report comparable enhancement factors as for the single color case.

Paper Details

Date Published: 25 February 2010
PDF: 12 pages
Proc. SPIE 7571, Single Molecule Spectroscopy and Imaging III, 75710A (25 February 2010); doi: 10.1117/12.840041
Show Author Affiliations
Heykel Aouani, Institut Fresnel, Aix-Marseille Univ., CNRS (France)
Nadia Djaker, Institut Fresnel, Aix-Marseille Univ., CNRS (France)
Jérôme Wenger, Institut Fresnel, Aix-Marseille Univ., CNRS (France)
Hervé Rigneault, Institut Fresnel, Aix-Marseille Univ., CNRS (France)


Published in SPIE Proceedings Vol. 7571:
Single Molecule Spectroscopy and Imaging III
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

© SPIE. Terms of Use
Back to Top