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Proceedings Paper

Dual-modal optical projection tomography microscopy for cancer diagnosis
Author(s): Qin Miao; Julia Yu; Michael G. Meyer; J. Richard Rahn; Thomas Neumann; Alan C. Nelson; Eric J. Seibel
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Paper Abstract

A dual-modal optical projection tomography microscope (OPTM) is presented, which produces three-dimensional images of single cells with isometric high resolution both in fluorescence and absorption mode. Depth of field of a high numerical aperture objective is extended by scanning the focal plane through the sample in order to enable reconstruction by back-projection method. Cells are fixed, stained, and mixed with optical gel and injected into the capillary for imaging. Combining absorption and fluorescence mode allows us to image different aspects of the disease process. Images of cells stained with both hematoxylin and fluorescence probes are shown. Registrations between two modes are discussed.

Paper Details

Date Published: 24 February 2010
PDF: 7 pages
Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700H (24 February 2010); doi: 10.1117/12.839824
Show Author Affiliations
Qin Miao, Univ. of Washington (United States)
Julia Yu, VisionGate Inc. (United States)
Michael G. Meyer, VisionGate Inc. (United States)
J. Richard Rahn, VisionGate Inc. (United States)
Thomas Neumann, VisionGate Inc. (United States)
Alan C. Nelson, VisionGate Inc. (United States)
Eric J. Seibel, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 7570:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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