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Proceedings Paper

Antigen detection at atomolar concentration using optical tweezers
Author(s): Mathieu Laliberté; François Bordeleau; Normand Marceau; Yunlong Sheng
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Paper Abstract

Methods that avoid intermediate amplification steps to detect protein markers of pathological disturbances would be of wide interest in the clinical environment. This is particularly the case in cancer diagnosis, where protein fragments are released into the blood by the emerging cancer cells. These fragments generate an antigen-antibody reaction, and the concentration of the antigen is known to modulate this interaction. Here we report on the development of a novel optical tweezers-based procedure to measure minute amount of antigen in a biological fluid. The force was applied on a 3μm polystyrene bead coated with Bovine Serum Albumin (BSA) attached on a 1.5 μm diameter borosilicate rod tip coated with anti-BSA antibody. First, we verified that the binding strength was dependent on the protein concentration on the bead. We then assessed the sensitivity range by finding the minimal BSA concentration in solution that can still interfere with the bead-rod linkage. On the whole, the results demonstrated that proteinous antigen present in a biological fluid could possibly be detectable at atomolar concentration through the use of an optical tweezers.

Paper Details

Date Published: 4 August 2009
PDF: 7 pages
Proc. SPIE 7386, Photonics North 2009, 738609 (4 August 2009); doi: 10.1117/12.839769
Show Author Affiliations
Mathieu Laliberté, COPL, Univ. Laval (Canada)
François Bordeleau, COPL, Univ. Laval (Canada)
Ctr. de recherché d l'Hôtel-Dieu de Québec (Canada)
Normand Marceau, Ctr. de recherché d l'Hôtel-Dieu de Québec (Canada)
Yunlong Sheng, COPL, Univ. Laval (Canada)

Published in SPIE Proceedings Vol. 7386:
Photonics North 2009
Réal Vallée, Editor(s)

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