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Proceedings Paper

Waveguide evanescent field fluorescence microscopy: waveguide mode scattering by non-uniform grating and defects in the wave guiding film
Author(s): Abdollah Hassanzadeh; Kenneth Kar Ho Wong
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Paper Abstract

Waveguide evanescent field fluorescence microscopy is an evanescent field based microscopy to visualize cell-substrate contact regions and solid thin films. Despite some advantages of this method compared to other evanescent field based microscopes, non-uniform illumination source and background are reported to be the problems for producing nonuniform images. We noticed similar non-uniformities in our primary results with ultra- thin solid film and cell-substrate contact regions. We used various microscopy methods to demonstrate induced inhomogenities in the waveguide evanescent field fluorescence microscopy images by secondary patterns in the gratings, and defects in waveguides surface. We discuss their effects on the waveguide microscopy background, ultra-thin solid films, and cell-substrate contact region images. Defect-free waveguides with uniform gratings fabricated and used to image phase separated lipids monolayer LB films and cell-substrate contact regions.

Paper Details

Date Published: 4 August 2009
PDF: 8 pages
Proc. SPIE 7386, Photonics North 2009, 73861P (4 August 2009); doi: 10.1117/12.839753
Show Author Affiliations
Abdollah Hassanzadeh, The Univ. of Western Ontario (Canada)
The Univ. of Kurdistan (Iran, Islamic Republic of)
Kenneth Kar Ho Wong, The Univ. of Western Ontario (Canada)

Published in SPIE Proceedings Vol. 7386:
Photonics North 2009
Réal Vallée, Editor(s)

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