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Proceedings Paper

Application of cytoplasmic Ca2+ fluorescence imaging techniques to study the molecular mechanisms of exercise-induced fatigue eliminated by Chinese medicine ginseng extract
Author(s): Yi Liu; Yanping Zhao; Heming Zhang; Songhao Liu
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Paper Abstract

The exercise-induced fatigue eliminated by Chinese medicine offers advantages including good efficiency and smaller side-effects, however, the exact mechanisms have not been classified. A lot of literatures indicated the cytosolic free Ca2+ concentrations of skeletal muscle cells increased significantly during exercise-induced fatigue. This study is aimed to establish a rat skeletal muscle cell model of exercise-induced fatigue. We applied cytoplasmic Ca2+ fluorescence imaging techniques to study the molecular mechanisms of exercise-induced fatigue eliminated by Chinese medicine ginseng extract. In our research, the muscle tissues from the newborn 3 days rats were taken out and digested into cells. The cells were randomly divided into the ginseng extract group and the control group. The cells from the two groups were cultured in the medium respectively added 2mg/ml ginseng extract and 2mg/ml D-hanks solution. After differentiating into myotubes, the two groups of cells treated with a fluorescent probe Fluo-3 AM were put on the confocal microscope and the fluorescence intensity of cells pre- and post- stimulation with dexamethasone were detected. It was found that cytoplasmic Ca2+ concentrations of the two groups of cells both increased post-stimulation, however, the increasing amplitude of fluorescence intensity of the ginseng extract group was significantly lower than that of the control group. In conclusion, stimulating the cells with dexamethasone is a kind of workable cell models of exercise-induced fatigue, and the molecular mechanisms of exercise-induced fatigue eliminated by ginseng extract may be connected to regulatating cytosolic free Ca2+ concentrations.

Paper Details

Date Published: 1 December 2009
PDF: 6 pages
Proc. SPIE 7507, 2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging, 75070L (1 December 2009); doi: 10.1117/12.838167
Show Author Affiliations
Yi Liu, South China Normal Univ. (China)
Yanping Zhao, South China Normal Univ. (China)
Heming Zhang, South China Normal Univ. (China)
Songhao Liu, South China Normal Univ. (China)


Published in SPIE Proceedings Vol. 7507:
2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging
Xiaocong Yuan; Yinmei Li; Arthur Chiou; Min Gu; Dennis Matthews; Colin Sheppard, Editor(s)

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