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Proceedings Paper

Light scattering modeling of bacteria using spheroids and cylinders
Author(s): Chunxia Feng; Lihua Huang; Jie Han; Guangchao Zhou; Aijun Zeng; Yongkai Zhao; Huijie Huang
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Paper Abstract

Numerical simulations of light scattering by irregularly shaped bacteria are carried out using the T-matrix method. A previously developed T-matrix code for the study of light scattering by randomly oriented non-spherical particles is used for the current purpose and it is validated against Mie-theory using coccus. Simplified particle shapes of spheroids and cylinders for simulating scattering by irregularly shaped bacteria are studied. The results for the angular distributions of the scattering matrix elements of B.Subtilis at wavelength 0.6328μm are presented. Their dependence on shape and model are discussed. Analysis suggests that spheroids perform better than cylinders for B.Subtilis. Calculations of the scatter matrix elements to determine bacteria sizes as well as shapes may be an accurate method and may be used to determine what the bacteria are.

Paper Details

Date Published: 20 November 2009
PDF: 10 pages
Proc. SPIE 7511, 2009 International Conference on Optical Instruments and Technology: Optoelectronic Measurement Technology and Systems, 75110P (20 November 2009); doi: 10.1117/12.837919
Show Author Affiliations
Chunxia Feng, Shanghai Institute of Optics and Fine Mechanics (China)
Graduate School of CAS (China)
Lihua Huang, Shanghai Institute of Optics and Fine Mechanics (China)
Jie Han, Shanghai Institute of Optics and Fine Mechanics (China)
Graduate School of CAS (China)
Guangchao Zhou, Shanghai Institute of Optics and Fine Mechanics (China)
Graduate School of CAS (China)
Aijun Zeng, Shanghai Institute of Optics and Fine Mechanics (China)
Yongkai Zhao, Shanghai Institute of Optics and Fine Mechanics (China)
Huijie Huang, Shanghai Institute of Optics and Fine Mechanics (China)


Published in SPIE Proceedings Vol. 7511:
2009 International Conference on Optical Instruments and Technology: Optoelectronic Measurement Technology and Systems
Shenghua Ye; Guangjun Zhang; Jun Ni, Editor(s)

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