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Proceedings Paper

Research on microfluidic chip and imaging system used to measure Ca2+ in cell
Author(s): Wei Zhou; Sixiang Zhang; Dugang Ran; Bao Liu
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Paper Abstract

The microfluidic fluorescence detecting system which is used to measure the concentration of Ca2+ had been designed. On the microfluidic chip we designed, cell-dyeing, cell fostering, reagent injecting and other operations can be completed. The monochromatic light came from optical monochromator which can emit continuous spectrum was used to excitated the fluorescent probe in the cell, then the fluorescence signal and image were sampled by the PMT and CCD, at last the data was processed and the content of Ca2+ in the cell was figured out by using the fluorescence ratio method. Meanwhile, by using the system, the dynamic curve of [Ca2+]1 in cell was given after being stimulated by high K+. The precise result verifies that the system is stable and credible and it meets the requirement of detecting [Ca2+]i in live cells in the filed of physiology.

Paper Details

Date Published: 6 August 2009
PDF: 9 pages
Proc. SPIE 7384, International Symposium on Photoelectronic Detection and Imaging 2009: Advances in Imaging Detectors and Applications, 73841A (6 August 2009); doi: 10.1117/12.835488
Show Author Affiliations
Wei Zhou, Hebei Univ. of Technology (China)
Sixiang Zhang, Hebei Univ. of Technology (China)
Dugang Ran, Hebei Univ. of Technology (China)
Bao Liu, Hebei Univ. of Technology (China)


Published in SPIE Proceedings Vol. 7384:
International Symposium on Photoelectronic Detection and Imaging 2009: Advances in Imaging Detectors and Applications
Kun Zhang; Xiang-jun Wang; Guang-jun Zhang; Ke-cong Ai, Editor(s)

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