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Proceedings Paper

A method for three-dimensional quantitative observation of the microstructure of biological samples
Author(s): Pengfei Wang; Dieyan Chen; Wanyun Ma; Hongxin Wu; Liang Ji; Jialin Sun; Danyu Lv; Lu Zhang; Ying Li; Ning Tian; Jinggao Zheng; Fengying Zhao
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Paper Abstract

Contemporary biology has developed into the era of cell biology and molecular biology, and people try to study the mechanism of all kinds of biological phenomena at the microcosmic level now. Accurate description of the microstructure of biological samples is exigent need from many biomedical experiments. This paper introduces a method for 3-dimensional quantitative observation on the microstructure of vital biological samples based on two photon laser scanning microscopy (TPLSM). TPLSM is a novel kind of fluorescence microscopy, which has excellence in its low optical damage, high resolution, deep penetration depth and suitability for 3-dimensional (3D) imaging. Fluorescent stained samples were observed by TPLSM, and afterward the original shapes of them were obtained through 3D image reconstruction. The spatial distribution of all objects in samples as well as their volumes could be derived by image segmentation and mathematic calculation. Thus the 3-dimensionally and quantitatively depicted microstructure of the samples was finally derived. We applied this method to quantitative analysis of the spatial distribution of chromosomes in meiotic mouse oocytes at metaphase, and wonderful results came out last.

Paper Details

Date Published: 31 August 2009
PDF: 6 pages
Proc. SPIE 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, 73824J (31 August 2009); doi: 10.1117/12.834098
Show Author Affiliations
Pengfei Wang, Tsinghua Univ. (China)
Dieyan Chen, Tsinghua Univ. (China)
Wanyun Ma, Tsinghua Univ. (China)
Hongxin Wu, Tsinghua Univ. (China)
Liang Ji, Tsinghua Univ. (China)
Jialin Sun, Tsinghua Univ. (China)
Danyu Lv, Peking Univ. Health Science Ctr. (China)
Lu Zhang, Peking Univ. Health Science Ctr. (China)
Ying Li, Peking Univ. Health Science Ctr. (China)
Ning Tian, Tsinghua Univ. (China)
Jinggao Zheng, Tsinghua Univ. (China)
Fengying Zhao, Tsinghua Univ. (China)


Published in SPIE Proceedings Vol. 7382:
International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging

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