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Proceedings Paper

Feature-based image analysis of zebrafish embryonic images
Author(s): Xiaoyan Xu; Xiaoyin Xu; Weiming Xia; Shunren Xia
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Paper Abstract

A pipeline of image analysis algorithm is developed for automatic analysis and quantification of neurons in microscopic images of zebrafish embryos. Key steps of pipeline include segmentation of zebrafish embryos from background, detection of the ROI, and quantitative measurement of neurons in the ROI. First, morphological operations are used to segment the zebrafish embryo from the background. Then based on the prior information that the torso has two approximately parallel boundaries corresponding to the back and abdomen, the algorithm automatically creates a ROI enclosing the torso. Finally, the number of neurons is obtained by improved Hough transform. Our results show that the image analysis algorithm has a high accuracy and fast computational speed. Development of such an automated image analysis pipeline represents a step toward high-throughput screening of zebrafish images with an accurate and reproducible quantification of neurons.

Paper Details

Date Published: 30 October 2009
PDF: 6 pages
Proc. SPIE 7495, MIPPR 2009: Automatic Target Recognition and Image Analysis, 74951I (30 October 2009); doi: 10.1117/12.832776
Show Author Affiliations
Xiaoyan Xu, Zhejiang Univ. (China)
Xiaoyin Xu, Brigham and Women's Hospital, Harvard Medical School (United States)
Weiming Xia, Brigham and Women's Hospital, Harvard Medical School (United States)
Shunren Xia, Zhejiang Univ. (China)

Published in SPIE Proceedings Vol. 7495:
MIPPR 2009: Automatic Target Recognition and Image Analysis
Tianxu Zhang; Bruce Hirsch; Zhiguo Cao; Hanqing Lu, Editor(s)

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