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Proceedings Paper

Determination of the optical properties of PNIPAAm gels used in biological applications
Author(s): A. Singh; A. E. Karsten; I. Mputle; A. Chetty; Kersch Naidoo
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Paper Abstract

Poly(N-isopropylacrylamide) i.e. PNIPAAm is a temperature sensitive smart material which displays a lower critical solution temperature (LCST) at 33-35°C. At the lower critical solution temperature, the gel changes from hydrophilic to hydrophobic which has significant consequences in cell culturing. The first known measurements of the optical properties i.e. absorption (μa) and reduced scattering (μ's) coefficients, as a function of temperature, of a series of crosslinked PNIPAAm gels, using an Integrating Sphere setup, is presented at a wavelength of 632.8 nm. These properties showed a direct correlation between the scattering coefficient and the crosslinker density for the gels. The absorption properties correlated well with the known absorption characteristics of these gels.

Paper Details

Date Published: 17 July 2009
PDF: 7 pages
Proc. SPIE 7373, Therapeutic Laser Applications and Laser-Tissue Interactions IV, 737317 (17 July 2009); doi: 10.1117/12.831882
Show Author Affiliations
A. Singh, Council for Scientific and Industrial Research (South Africa)
A. E. Karsten, Council for Scientific and Industrial Research (South Africa)
I. Mputle, Council for Scientific and Industrial Research (South Africa)
A. Chetty, Council for Scientific and Industrial Research (South Africa)
Kersch Naidoo, Council for Scientific and Industrial Research (South Africa)


Published in SPIE Proceedings Vol. 7373:
Therapeutic Laser Applications and Laser-Tissue Interactions IV
Ronald Sroka; Lothar D. Lilge, Editor(s)

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