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Proceedings Paper

Evaluation of the PDT effect of Foscan and Fospeg in the LNCaP human prostate cancer cell line
Author(s): A. Petri; M. Kyriazi; E. Alexandratou; M. Rallis; S. Gräfe; D. Yova
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Paper Abstract

In this paper the cytotoxic effect of m-THPC, Foscan®, as well as of the liposomal formulation of m-THPC, Fospeg®,(kind offer of Biolitec) were studied post PDT in the human prostate cancer cell line LNCaP. The cells were incubated for 24h with 0.15 μg/ml and 1.2 μg/ml Foscan® and Fospeg®. Irradiation was performed with a 652nm laser and energy doses 180, 360 and 540mJ/cm2. The effect was assessed by the MTT viability test 24h after irradiation. Also the intracellular localization of Foscan® and Fospeg® was monitored by using Laser Scanning Confocal Microscopy Imaging. The results showed no dark toxicity either with Foscan® or Fospeg® at any concentration. Also irradiation at each energy dose in the absence of any photosensitizer, did not affect cellular viability. The cellular death caused after Photodynamic Treatment was dependent on m-THPC concentration and formulation, as well as the delivered energy dose. Fospeg® was more effective as LD50 was achieved with 0.15μg/ml at 180mJ/cm2 while for the same cytotoxic result 1.2μg/ml Foscan® was needed. Images from confocal microscopy revealed higher fluorescence intensity in the cytoplasm after incubation with Fospeg®, than upon incubation with Foscan® under the same experimental conditions.

Paper Details

Date Published: 17 July 2009
PDF: 8 pages
Proc. SPIE 7373, Therapeutic Laser Applications and Laser-Tissue Interactions IV, 73731I (17 July 2009); doi: 10.1117/12.831881
Show Author Affiliations
A. Petri, National Technical Univ. of Athens (Greece)
M. Kyriazi, National Technical Univ. of Athens (Greece)
E. Alexandratou, National Technical Univ. of Athens (Greece)
M. Rallis, National and Kapodistrian Univ. of Athens (Greece)
S. Gräfe, biolitec AG (Germany)
D. Yova, National Technical Univ. of Athens (Greece)


Published in SPIE Proceedings Vol. 7373:
Therapeutic Laser Applications and Laser-Tissue Interactions IV
Ronald Sroka; Lothar D. Lilge, Editor(s)

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