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Proceedings Paper

In vivo in situ en face optical coherence tomography imaging of chick embryos
Author(s): Michael Leitner; Joana Castanheira; Luís Ferreira; Mónica Ferreira; Isabel Palmeirim; Carla C. Rosa; Adrian Gh. Podoleanu
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Paper Abstract

Chick embryos are among the most studied species in development biology because they are easily obtained, highly accessible and present a similar development to that of humans. Normally morphological studies are carried out with confocal microscopy, however in-situ imaging is impossible and in- vivo imaging can only be performed with great difficulty. For confocal microscopy the embryo has to be studied outside the egg, what generally also means a short life expectancy of the embryo. Additionally, extracting the embryo of the egg precludes the possibility of studying its development in its natural environment. In this paper it is shown that en-face optical coherence tomography (en-face OCT) is a possible solution to overcome these difficulties allowing for an in-situ and in-vivo study over a timescale of several days. With en-face OCT it is possible to accompany the development of one single embryo over several days and to acquire high resolution and axially resolved images.

Paper Details

Date Published: 13 July 2009
PDF: 7 pages
Proc. SPIE 7372, Optical Coherence Tomography and Coherence Techniques IV, 73720Y (13 July 2009); doi: 10.1117/12.831859
Show Author Affiliations
Michael Leitner, Univ. of Porto (Portugal)
INESC Porto (Portugal)
Univ. of Kent at Canterbury (United Kingdom)
Joana Castanheira, Univ. of Minho (Portugal)
Luís Ferreira, Univ. of Minho (Portugal)
Mónica Ferreira, Univ. of Minho (Portugal)
Isabel Palmeirim, Univ. of Minho (Portugal)
Carla C. Rosa, Univ. of Porto (Portugal)
INESC Porto (Portugal)
Adrian Gh. Podoleanu, Univ. of Kent at Canterbury (United Kingdom)

Published in SPIE Proceedings Vol. 7372:
Optical Coherence Tomography and Coherence Techniques IV
Peter E. Andersen; Brett E. Bouma, Editor(s)

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