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Proceedings Paper

Turbid polarimetry for tissue characterization
Author(s): Michael F. G. Wood; Nirmalya Ghosh; Marika A. Wallenburg; Eduardo H. Moriyama; Shu-Hong Li; Richard D. Weisel; Brian C. Wilson; Ren-Ke Li; I. Alex Vitkin
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Paper Abstract

We have developed a novel turbid polarimetry platform for characterization of biological tissues. Currently, we are exploring the use of this platform for characterization of the extracellular matrix particularly for use in monitoring regenerative treatments of myocardial infarctions. Collagen is a fibrous protein and exhibits birefringence due to different refractive indices parallel and perpendicular to the direction of the fibers. As a result, changes in the collagen content and organization in the tissue lead to changes in birefringence. We demonstrate our ability to measure these extracellular changes in vivo using a mouse dorsal window chamber model. Collagenase was injected into a region of the chamber to denature the extracellular matrix. Birefringence measurements show a large decrease in birefringence associated with the destruction of collagen fibers. Birefringence measurements were also made through ex vivo myocardial tissues from rats with induced myocardial infarctions including a number that had undergone regenerative treatment with mesenchymal stem cells. Results show a decrease in birefringence from normal to infracted myocardium, indicating a decrease in tissue organization associated with scar formation, however, an increase in birefringence was seen in those myocardial tissues that had undergone regenerative treatment indicating reorganization of tissue structure.

Paper Details

Date Published: 10 July 2009
PDF: 10 pages
Proc. SPIE 7371, Novel Optical Instrumentation for Biomedical Applications IV, 737106 (10 July 2009); doi: 10.1117/12.831744
Show Author Affiliations
Michael F. G. Wood, Ontario Cancer Institute, Univ. of Toronto (Canada)
Nirmalya Ghosh, Ontario Cancer Institute, Univ. of Toronto (Canada)
Marika A. Wallenburg, Ontario Cancer Institute, Univ. of Toronto (Canada)
Eduardo H. Moriyama, Ontario Cancer Institute, Univ. of Toronto (Canada)
Shu-Hong Li, Toronto General Research Institute, Canada and Toronto General Hospital (Canada)
Richard D. Weisel, Toronto General Research Institute, Canada and Toronto General Hospital (Canada)
Brian C. Wilson, Ontario Cancer Institute, Univ. of Toronto (Canada)
Ren-Ke Li, Toronto General Research Institute, Canada and Toronto General Hospital (Canada)
I. Alex Vitkin, Ontario Cancer Institute, Univ. of Toronto (Canada)


Published in SPIE Proceedings Vol. 7371:
Novel Optical Instrumentation for Biomedical Applications IV
Christian D. Depeursinge; I. Alex Vitkin, Editor(s)

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