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Proceedings Paper

Multiplexed diagnostics and spectroscopic ruler applications with terbium to quantum dots FRET
Author(s): Daniel Geissler; Nathaniel G. Butlin; Diana Hill; Hans-Gerd Löhmannsröben; Niko Hildebrandt
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Paper Abstract

In this contribution we present the application of five different commercially available semiconductor core/shell quantum dots (Qdot® Nanocrystals - Invitrogen Corp.) as multiplexing FRET acceptors together with a commercial supramolecular terbium complex (Lumi4®-Tb - Lumiphore Inc.) as donor in a homogeneous immunoassay format. To realize the molecular recognition necessary for a FRET assay, the terbium complex was labeled to streptavidin (sAv- Lumi4-Tb) and the QDs were surface functionalized with biotin (Biot-QD). The biotin-streptavidin binding serves as a proof-of-principle representative for many biological interactions taking place on the nanometer scale (e.g. immunoassays). The presented FRET system can be efficiently used for the detection of inter- and intramolecular processes for clinical diagnostics and biomedical spectroscopy as well as molecular ruler applications and microscopy.

Paper Details

Date Published: 7 July 2009
PDF: 8 pages
Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 73680P (7 July 2009); doi: 10.1117/12.831631
Show Author Affiliations
Daniel Geissler, Univ. Potsdam (Germany)
Nathaniel G. Butlin, Lumiphore Inc. (United States)
Diana Hill, Univ. Potsdam (Germany)
Hans-Gerd Löhmannsröben, Univ. Potsdam (Germany)
Niko Hildebrandt, Fraunhofer Institute for Applied Polymer Research (Germany)

Published in SPIE Proceedings Vol. 7368:
Clinical and Biomedical Spectroscopy
Irene Georgakoudi; Jürgen Popp; Katarina Svanberg, Editor(s)

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