Share Email Print
cover

Proceedings Paper

Multiplexed diagnostics and spectroscopic ruler applications with terbium to quantum dots FRET
Author(s): Daniel Geissler; Nathaniel G. Butlin; Diana Hill; Hans-Gerd Löhmannsröben; Niko Hildebrandt
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

In this contribution we present the application of five different commercially available semiconductor core/shell quantum dots (Qdot® Nanocrystals - Invitrogen Corp.) as multiplexing FRET acceptors together with a commercial supramolecular terbium complex (Lumi4®-Tb - Lumiphore Inc.) as donor in a homogeneous immunoassay format. To realize the molecular recognition necessary for a FRET assay, the terbium complex was labeled to streptavidin (sAv- Lumi4-Tb) and the QDs were surface functionalized with biotin (Biot-QD). The biotin-streptavidin binding serves as a proof-of-principle representative for many biological interactions taking place on the nanometer scale (e.g. immunoassays). The presented FRET system can be efficiently used for the detection of inter- and intramolecular processes for clinical diagnostics and biomedical spectroscopy as well as molecular ruler applications and microscopy.

Paper Details

Date Published: 7 July 2009
PDF: 8 pages
Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 73680P (7 July 2009); doi: 10.1117/12.831631
Show Author Affiliations
Daniel Geissler, Univ. Potsdam (Germany)
Nathaniel G. Butlin, Lumiphore Inc. (United States)
Diana Hill, Univ. Potsdam (Germany)
Hans-Gerd Löhmannsröben, Univ. Potsdam (Germany)
Niko Hildebrandt, Fraunhofer Institute for Applied Polymer Research (Germany)


Published in SPIE Proceedings Vol. 7368:
Clinical and Biomedical Spectroscopy
Irene Georgakoudi; Jürgen Popp; Katarina Svanberg, Editor(s)

© SPIE. Terms of Use
Back to Top