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Proceedings Paper

Spatially resolved bimodal spectroscopy for classification/evaluation of mouse skin inflammatory and pre-cancerous stages
Author(s): Gilberto Díaz-Ayil; Marine Amouroux; Fabien Clanché; Yves Granjon; Walter C. P. M. Blondel
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Paper Abstract

Spatially-resolved bimodal spectroscopy (multiple AutoFluorescence AF excitation and Diffuse Reflectance DR), was used in vivo to discriminate various healthy and precancerous skin stages in a pre-clinical model (UV-irradiated mouse): Compensatory Hyperplasia CH, Atypical Hyperplasia AH and Dysplasia D. A specific data preprocessing scheme was applied to intensity spectra (filtering, spectral correction and intensity normalization), and several sets of spectral characteristics were automatically extracted and selected based on their discrimination power, statistically tested for every pair-wise comparison of histological classes. Data reduction with Principal Components Analysis (PCA) was performed and 3 classification methods were implemented (k-NN, LDA and SVM), in order to compare diagnostic performance of each method. Diagnostic performance was studied and assessed in terms of Sensibility (Se) and Specificity (Sp) as a function of the selected features, of the combinations of 3 different inter-fibres distances and of the numbers of principal components, such that: Se and Sp ≈ 100% when discriminating CH vs. others; Sp ≈ 100% and Se > 95% when discriminating Healthy vs. AH or D; Sp ≈ 74% and Se ≈ 63% for AH vs. D.

Paper Details

Date Published: 7 July 2009
PDF: 8 pages
Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 73680F (7 July 2009); doi: 10.1117/12.831628
Show Author Affiliations
Gilberto Díaz-Ayil, Ctr. de Recherche en Automatique de Nancy, CNRS, Nancy-Univ. (France)
Marine Amouroux, Ctr. de Recherche en Automatique de Nancy, CNRS, Nancy-Univ. (France)
Fabien Clanché, Ctr. de Recherche en Automatique de Nancy, CNRS, Nancy-Univ. (France)
Yves Granjon, Ctr. de Recherche en Automatique de Nancy, CNRS, Nancy-Univ. (France)
Walter C. P. M. Blondel, Ctr. de Recherche en Automatique de Nancy, CNRS, Nancy-Univ. (France)


Published in SPIE Proceedings Vol. 7368:
Clinical and Biomedical Spectroscopy
Irene Georgakoudi; Jürgen Popp; Katarina Svanberg, Editor(s)

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