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Proceedings Paper

Discrimination of microorganisms and cells in tissue engineering by Raman spectroscopy
Author(s): Steffen Koch; Marieke Dreiling; Matthias Gutekunst; Carsten Bolwien; Hagen Thielecke; Heike Mertsching
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Paper Abstract

Sterility testing of cell or tissue cultures is an essential task in the fields of regenerative medicine and tissue engineering. Especially in case of Good manufacturing practice (GMP) of cell and tissue based transplants. We present a system based on a commercially available microscope equipped with a microfluidic cell that prepares the particles found in the solution for analysis. A Raman-spectrometer attachment optimized for non-destructive, rapid recording of Raman spectra, and a data acquisition and analysis tool for identification of the particles. Identification of critical particles like microorganisms via microscopic imaging and subsequent image analysis is carried out before micro-Raman analysis of those particles is then carried out with an excitation wavelength of 785 nm. However an automated image analysis of small particles from supernatant of biopsies on a filter chip with tiny holes is a difficult task. Especially for the discrimination of small particles like cell debris and bacteria, which have a quite similar range of size. Because of that particles in the supernatant and microorganisms have to be discriminated by means of Raman spectroscopy. We present here a Raman based method to discriminate between cells, microorganisms and particles in cell culture.

Paper Details

Date Published: 8 July 2009
PDF: 10 pages
Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 736829 (8 July 2009); doi: 10.1117/12.831599
Show Author Affiliations
Steffen Koch, Fraunhofer Institute for Interfacial Engineering and Biotechnology (Germany)
Marieke Dreiling, Fraunhofer Institute for Interfacial Engineering and Biotechnology (Germany)
Matthias Gutekunst, Fraunhofer Institute for Interfacial Engineering and Biotechnology (Germany)
Carsten Bolwien, Fraunhofer Institute for Physical Measurement Techniques (Germany)
Hagen Thielecke, Fraunhofer Institute for Biomedical Engineering (Germany)
Heike Mertsching, Fraunhofer Institute for Interfacial Engineering and Biotechnology (Germany)


Published in SPIE Proceedings Vol. 7368:
Clinical and Biomedical Spectroscopy
Irene Georgakoudi; Jürgen Popp; Katarina Svanberg, Editor(s)

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