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Proceedings Paper

Darkfield scattering spectroscopic microscopy evaluation using polystyrene beads
Author(s): Michael Schmitz; René Michels; Alwin Kienle
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Paper Abstract

Diameters of single polystyrene beads were determined within ~10 nm accuracy by comparing Mie theory oscillations and wavelength resolved measurements. The setup is realized with an axicon supported reflected darkfield microscope and is herein presented in detail. Further we explain a theoretical model considering the effective numerical aperature of the measuring system. A fitting algorithm allows rapid characterization of the sphere diameters.

Paper Details

Date Published: 7 July 2009
PDF: 6 pages
Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 73681W (7 July 2009); doi: 10.1117/12.831591
Show Author Affiliations
Michael Schmitz, Institut für Lasertechnologien in der Medizin und Meßtechnik (Germany)
René Michels, Institut für Lasertechnologien in der Medizin und Meßtechnik (Germany)
Alwin Kienle, Institut für Lasertechnologien in der Medizin und Meßtechnik (Germany)


Published in SPIE Proceedings Vol. 7368:
Clinical and Biomedical Spectroscopy
Irene Georgakoudi; Jürgen Popp; Katarina Svanberg, Editor(s)

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