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Proceedings Paper

A membrane-associated FRET sensor for detection of apoptosis
Author(s): Herbert Schneckenburger; Michael Wagner; Petra Weber; Thomas Bruns; Heiko Steuer; Brigitte Angres
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Paper Abstract

A membrane associated caspase sensor based on Förster Resonance Energy Transfer (FRET) between enhanced cyan fluorescent protein (Mem-ECFP) and yellow fluorescent protein (EYFP) is reported. Upon apoptosis a caspase sensitive amino acid peptide linker (DEVD) between these proteins is cleaved, and pronounced changes of fluorescence spectra and lifetimes are observed. Membrane selective detection of fluorescent proteins in cultivated HeLa cervix carcinoma cells is achieved by total internal reflection fluorescence microscopy (TIRFM) with high sensitivity and resolution.

Paper Details

Date Published: 8 July 2009
PDF: 6 pages
Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 73680Q (8 July 2009); doi: 10.1117/12.831570
Show Author Affiliations
Herbert Schneckenburger, Hochschule Aalen, Institut für Angewandte Forschung (Germany)
Michael Wagner, Hochschule Aalen, Institut für Angewandte Forschung (Germany)
Petra Weber, Hochschule Aalen, Institut für Angewandte Forschung (Germany)
Thomas Bruns, Hochschule Aalen, Institut für Angewandte Forschung (Germany)
Heiko Steuer, Naturwissenschaftliches und Medizinisches Institut an der Univ. Tübingen (Germany)
Brigitte Angres, Naturwissenschaftliches und Medizinisches Institut an der Univ. Tübingen (Germany)


Published in SPIE Proceedings Vol. 7368:
Clinical and Biomedical Spectroscopy
Irene Georgakoudi; Jürgen Popp; Katarina Svanberg, Editor(s)

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