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Proceedings Paper

FRET detection for neurobiological applications using a total internal reflection fluorescence lifetime imaging microscope
Author(s): V. Devauges; P. Blandin; J. C. Cossec; S. Lécart; C. Marquer; M. C. Potier; F. Druon; P. Georges; S. Lévêque-Fort
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Paper Abstract

We present the development of a time resolved TIRF microscope illuminated by a supercontinuum laser source. It permits to perform wide-field fluorescence lifetime imaging of neurobiological processes at the plasma membrane with subwavelength axial resolution.

Paper Details

Date Published: 1 July 2009
PDF: 7 pages
Proc. SPIE 7367, Advanced Microscopy Techniques, 736711 (1 July 2009); doi: 10.1117/12.831537
Show Author Affiliations
V. Devauges, Lab. de Photophysique Moléculaire, CNRS, Univ. Paris Sud (France)
Lab. Charles Fabry de l'Institut d'Optique, CNRS, Univ. Paris Sud, (France)
Univ. Paris Sud (France)
P. Blandin, Lab. de Photophysique Moléculaire, CNRS, Univ. Paris Sud (France)
Lab. Charles Fabry de l'Institut d'Optique, CNRS, Univ. Paris Sud, (France)
Univ. Paris Sud (France)
J. C. Cossec, CRICM, CNRS (France)
S. Lécart, Univ. Paris Sud (France)
C. Marquer, CRICM, CNRS (France)
M. C. Potier, CRICM, CNRS (France)
F. Druon, Lab. Charles Fabry de l'Institut d'Optique, CNRS, Univ. Paris Sud (France)
Univ. Paris Sud (France)
P. Georges, Lab. Charles Fabry de l'Institut d'Optique, CNRS, Univ. Paris Sud, (France)
Univ. Paris Sud (France)
S. Lévêque-Fort, Lab. de Photophysique Moléculaire, CNRS, Univ. Paris Sud (France)
Univ. Paris Sud (France)


Published in SPIE Proceedings Vol. 7367:
Advanced Microscopy Techniques
Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally, Editor(s)

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