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Proceedings Paper

Barium titanate nanoparticles used as second harmonic radiation imaging probes for cell imaging
Author(s): Chia-Lung Hsieh; Rachel Grange; Ye Pu; Demetri Psaltis
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Paper Abstract

Luminescent markers play a key role in imaging techniques for life science since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time and broad flexibility in the choice of excitation wavelength. In this paper, we report the use of nanoparticles for cell imaging. We first stabilized the BaTiO3 nanoparticles in suspension and characterized the optical properties. We also demonstrated the functionalization of BaTiO3 nanoparticles by conjugating IgG antibody on the surface of the nanoparticles. These functionalized nanocrystals are capable of specific labeling the antigens of interest.

Paper Details

Date Published: 1 July 2009
PDF: 6 pages
Proc. SPIE 7367, Advanced Microscopy Techniques, 73670D (1 July 2009); doi: 10.1117/12.831500
Show Author Affiliations
Chia-Lung Hsieh, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
California Institute of Technology (United States)
Rachel Grange, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Ye Pu, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
California Institute of Technology (United States)
Demetri Psaltis, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
California Institute of Technology (United States)


Published in SPIE Proceedings Vol. 7367:
Advanced Microscopy Techniques
Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally, Editor(s)

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