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Proceedings Paper

Controlling the emission of organic dyes for high sensitivity and super-resolution microscopy
Author(s): Thorben Cordes; Ingo H. Stein; Carsten Forthmann; Christian Steinhauer; Monika Walz; Wolfram Summerer; Britta Person; Jan Vogelsang; Philip Tinnefeld
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Paper Abstract

In this paper we show that the emission of ordinary organic dyes can be controlled in order to increase photostability or to induce long off-states for superresolution microscopy. We therefore extend a recently introduced concept that utilizes triplet-state quenching via redox-reactions and recovery of the electronic ground-state by complementary redoxreactions: it is shown that different reagents in an oxidizing and reducing system (ROXS) can positively influence the fluorescence properties of organic dyes. In more detail, the effects of Trolox, a ferrocene-based compound, an oxidized quinone derivative of Trolox and nitrobenzoic acid are investigated and compared to the prototypical compounds ascorbic acid and N,N methylviologen. While the redox potential is the most important parameter for the realization of the ROXS concept it is demonstrated that also kinetic aspects have to be taken into account to explain the properties of the specific redox agents. Photostabilization and the induction of off-states are of paramount importance for fluorescence microscopy in general and especially for superresolution microscopy based on "blinking" molecules.

Paper Details

Date Published: 1 July 2009
PDF: 9 pages
Proc. SPIE 7367, Advanced Microscopy Techniques, 73671D (1 July 2009); doi: 10.1117/12.831495
Show Author Affiliations
Thorben Cordes, Ludwig-Maximilians-Univ. (Germany)
Ingo H. Stein, Ludwig-Maximilians-Univ. (Germany)
Carsten Forthmann, Ludwig-Maximilians-Univ. (Germany)
Christian Steinhauer, Ludwig-Maximilians-Univ. (Germany)
Monika Walz, Ludwig-Maximilians-Univ. (Germany)
Wolfram Summerer, Ludwig-Maximilians-Univ. (Germany)
Britta Person, Ludwig-Maximilians-Univ. (Germany)
Jan Vogelsang, Ludwig-Maximilians-Univ. (Germany)
Philip Tinnefeld, Ludwig-Maximilians-Univ. (Germany)

Published in SPIE Proceedings Vol. 7367:
Advanced Microscopy Techniques
Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally, Editor(s)

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