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Proceedings Paper

Live cell imaging with surface plasmon-mediated fluorescence microscopy
Author(s): Karla Balaa; Viviane Devauges; Yannick Goulam; Vincent Studer; Sandrine Lévêque-Fort; Emmanuel Fort
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Paper Abstract

We present a new imaging technique using surface-plasmon mediated fluorescence microscopy. It uses a similar configuration as standard prismless Total Internal Reflection Fluorescence Microscopy with an additional metallic thin film. In the case of a silver thin film we show that this technique offers many advantages: distance dependence emission filter for improved signal to noise ratio and enhanced molecular detection efficiency. This technique is of particular interest in membrane and adhesion imaging. We present real time images on live cells.

Paper Details

Date Published: 2 July 2009
PDF: 8 pages
Proc. SPIE 7367, Advanced Microscopy Techniques, 736710 (2 July 2009); doi: 10.1117/12.831493
Show Author Affiliations
Karla Balaa, Institut Langevin, CNRS, Univ. Paris Diderot (France)
Viviane Devauges, Lab. de PhotoPhysique Moléculaire, CNRS, Univ. Paris-Sud (France)
Yannick Goulam, Institut Langevin, CNRS, Univ. Paris Diderot (France)
Vincent Studer, Ecole Supérieure de Physique et de Chimie Industrielles (France)
Sandrine Lévêque-Fort, Lab. de PhotoPhysique Moléculaire, CNRS, Univ. Paris-Sud (France)
Emmanuel Fort, Institut Langevin, CNRS, Univ. Paris Diderot (France)


Published in SPIE Proceedings Vol. 7367:
Advanced Microscopy Techniques
Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally, Editor(s)

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