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Proceedings Paper

Contrast enhancement in second harmonic imaging: discriminating between muscle and collagen
Author(s): Sotiris Psilodimitrakopoulos; David Artigas; Guadalupe Soria; Ivan Amat-Roldan; Iratxe Torre; Eduard Gratacos; Anna M. Planas; Pablo Loza-Alvarez
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Paper Abstract

In this study, polarization second harmonic generation (SHG) imaging is used and data analysis is developed to gain contrast and to discriminate with pixel resolution, in the same image, SHG source architectures. We use mammalian tissue in which both skeletal muscle and fibrilar collagen can be found. The images are fitted point by point using an algorithm based on a biophysical model, where the coefficient of determination is utilized as a filtering mechanism. For the whole image we retrieve for every pixel, the effective orientation, θe , of the SHG active structures. As a result a new image is formed which its contrast depends on the values of θe . Collagen presented in the forward direction for a predefined region of interest (ROI), peak distribution of angles θe centered in the region of ~45°, while muscle in the region of ~65°. Consequently, collagen and muscle are represented in different colors in the same image. Thus, here we show that it is possible to gain contrast and to discriminate between collagen and muscle without the use of any exogenous labeling or any co-localization with fluorescence imaging.

Paper Details

Date Published: 1 July 2009
PDF: 8 pages
Proc. SPIE 7367, Advanced Microscopy Techniques, 73670S (1 July 2009); doi: 10.1117/12.831487
Show Author Affiliations
Sotiris Psilodimitrakopoulos, ICFO-Instituto de Ciencias Fotónicas (Spain)
David Artigas, ICFO-Instituto de Ciencias Fotónicas (Spain)
Univ. Politècnica de Catalunya (Spain)
Guadalupe Soria, IIBB-Institut d'Investigacions Biomèdiques de Barcelona (Spain)
Ivan Amat-Roldan, ICFO-Instituto de Ciencias Fotónicas (Spain)
Hospital Clinic-IDIBAPS and CIBER-ER (Spain)
Iratxe Torre, Hospital Clinic-IDIBAPS and CIBER-ER (Spain)
Eduard Gratacos, Hospital Clinic-IDIBAPS and CIBER-ER (Spain)
Anna M. Planas, IIBB-Institut d'Investigacions Biomèdiques de Barcelona (Spain)
Pablo Loza-Alvarez, ICFO-Instituto de Ciencias Fotónicas (Spain)

Published in SPIE Proceedings Vol. 7367:
Advanced Microscopy Techniques
Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally, Editor(s)

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