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Proceedings Paper

Reflective confocal laser scanning microscopy and nonlinear microscopy of cross-linked rabbit cornea
Author(s): Alexander Krueger; Marina Hovakimyan; Diego F. Ramirez; Oliver Stachs; Rudolf F. Guthoff; Alexander Heisterkamp
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Paper Abstract

Cross-linking of the cornea with application of Ribovlavin and UV-A light is an evolving clinical treatment of the eye disease keratoconus. Despite the positive clinical track record of corneal cross-linking, the complex wound healing process after the treatment is still under investigation. In this study an animal model was used to clarify the state of wound healing 5 weeks after treatment. Cross-linked rabbit corneae were imaged with reflective confocal laser scanning and nonlinear microscopy, namely second harmonic imaging microscopy (SHIM) and two-photon excited autofluorescence. First results show that the NAD(P) H-autofluorescence of the corneal keratocytes and their scattering signal still show a signature of the treatment five weeks after the cross-linking procedure. The SHIM signals show the structural morphology of the fibrous collagen sheets in the stroma of the cornea. SHIM detected in the forward direction differs substantially from backward SHIM, but no signature of treatment was found in both detection channels of the SHIM signal.

Paper Details

Date Published: 1 July 2009
PDF: 9 pages
Proc. SPIE 7367, Advanced Microscopy Techniques, 736719 (1 July 2009); doi: 10.1117/12.831478
Show Author Affiliations
Alexander Krueger, Laser Zentrum Hannover e.V. (Germany)
Marina Hovakimyan, Universitaetsaugenklinik Rostock (Germany)
Diego F. Ramirez, Laser Zentrum Hannover e.V. (Germany)
Oliver Stachs, Universitaetsaugenklinik Rostock (Germany)
Rudolf F. Guthoff, Universitaetsaugenklinik Rostock (Germany)
Alexander Heisterkamp, Laser Zentrum Hannover e.V. (Germany)

Published in SPIE Proceedings Vol. 7367:
Advanced Microscopy Techniques
Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally, Editor(s)

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