Share Email Print
cover

Proceedings Paper

Application of color digital holographic microscopy for analysis of stained tissue sections
Author(s): Xiaoli Mo; Björn Kemper; Patrik Langehanenberg; Angelika Vollmer; Jinghui Xie; Gert von Bally
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

The analysis of stained tissue sections represents an important tool in medical diagnostics. Color digital holographic microscopy offers subsequent multi-focus true color imaging with simultaneous quantitative phase contrast analysis. Investigations on digital recording and numerical reconstructions of color digital holographic images have been performed by applying a transmission microscope experimental setup in holographic off-axis geometry. Three monochromatic digital holograms with different wavelengths in red, green and blue spectral range are recorded. After digital holographic refocusing and compensation for aberrations of the microscope imaging system by digital image correlation the numerically reconstructed amplitude distributions are combined into color images. The applicability of the method is demonstrated by results obtained from stained intestine tissue sections.

Paper Details

Date Published: 1 July 2009
PDF: 6 pages
Proc. SPIE 7367, Advanced Microscopy Techniques, 736718 (1 July 2009); doi: 10.1117/12.831476
Show Author Affiliations
Xiaoli Mo, Ctr. for Biomedical Optics and Photonics, Univ. of Muenster (Germany)
Beijing Institute of Technology (China)
Björn Kemper, Ctr. for Biomedical Optics and Photonics, Univ. of Muenster (Germany)
Patrik Langehanenberg, Ctr. for Biomedical Optics and Photonics, Univ. of Muenster (Germany)
Angelika Vollmer, Ctr. for Biomedical Optics and Photonics, Univ. of Muenster (Germany)
Jinghui Xie, Beijing Institute of Technology (China)
Gert von Bally, Ctr. for Biomedical Optics and Photonics, Univ. of Muenster (Germany)


Published in SPIE Proceedings Vol. 7367:
Advanced Microscopy Techniques
Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally, Editor(s)

© SPIE. Terms of Use
Back to Top