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Proceedings Paper

Orientation determination of single molecules by highly focused generalized cylindrical vector beams
Author(s): Xianghui Wang; Shengjiang Chang; Lie Lin; Linrui Wang; Shujuan Hao
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Paper Abstract

The orientation of the dipole moment, which is one of important parameters in single-molecule fluorescence spectroscopy, is determined by highly focused generalized cylindrical vector beams combined with a confocal far-field microscope. A generalized cylindrical vector beam can be decomposed into a linear superposition of radially polarized and azimuthally polarized components and the ratio of the radial and longitudinal components of the focal electric field can be adjusted by changing the polarization direction. As compared to the case of a radially polarized incident beam, numerical results demonstrate that fluorescence intensity difference among single molecules with different orientations can be effectively overcome for a particular polarization direction of the generalized cylindrical vector beam, which may increases the signal-to-noise ratio of the practical experiments and then ease the difficulty in orientation determination of single molecules.

Paper Details

Date Published: 28 August 2009
PDF: 6 pages
Proc. SPIE 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, 738239 (28 August 2009); doi: 10.1117/12.830689
Show Author Affiliations
Xianghui Wang, Nankai Univ. (China)
Shengjiang Chang, Nankai Univ. (China)
Lie Lin, Nankai Univ. (China)
Linrui Wang, Nankai Univ. (China)
Shujuan Hao, Nankai Univ. (China)


Published in SPIE Proceedings Vol. 7382:
International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging
Farzin Amzajerdian; Chun-qing Gao; Tian-yu Xie, Editor(s)

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