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Proceedings Paper

Digital holographic microscopy for quantitative phase-contrast imaging
Author(s): Huaying Wang; Rui Han; Zhiwei Tang
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Paper Abstract

In this paper, a simple experimental setup with an off-axis lenless Fourier arrangement without pre-magnification is built. Based on common recording conditions of digital hologram, an improved recording condition is proposed, with which higher resolution of reconstructed image can be obtained. By using a USAF test target as microscopic object, the digital hologram is recorded by a "SensiCam" CCD sensor, which has an array of 1317×1035 pixels with 6.8μm x 6.8μm pixel size. Based on Fresnel diffraction formula, the recorded hologram is reconstructed numerically. The lateral resolution of 2.76 μm without any pre-magnification has been demonstrated experimentally. The corresponding theoretical resolution limitation is 2.39 μm. The result matches the theoretical prediction very well. In order to reconstruct three-dimensional information, the recording parameters, such as the recording distance and the offset of the reference point source, are determined accurately by using a synthetic method of an automatic phase correction procedure and a manual adjustment process. Then, the accurate phase image can be obtained. The axial resolution better than 10nm has been also demonstrated experimentally.

Paper Details

Date Published: 20 May 2009
PDF: 6 pages
Proc. SPIE 7283, 4th International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment, 72833P (20 May 2009); doi: 10.1117/12.828783
Show Author Affiliations
Huaying Wang, Hebei Univ. of Engineering (China)
Rui Han, Hebei Univ. of Engineering (China)
Zhiwei Tang, Hebei Univ. of Engineering (China)


Published in SPIE Proceedings Vol. 7283:
4th International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment
Yudong Zhang; James C. Wyant; Robert A. Smythe; Hexin Wang, Editor(s)

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