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Proceedings Paper

Loss of intercellular adhesion leads to differential accumulation of hypericin in bladder cancer
Author(s): S. Sasidharan Lucky; Ramaswamy Bhuvaneswari; William W. L. Chin; Weber K. O. Lau; Malini C. D. Olivo
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Paper Abstract

Photodynamic diagnosis (PDD) exploits the photoactive nature of certain compounds, namely photosensitizers, in order to enhance the visual demarcation between normal and neoplastic tissue. Hypericin is one such potent photosensitizer that preferentially accumulate in neoplastic tissue, and fluoresce in the visible spectrum when illuminated with light of an appropriate wavelength. In our study, we investigated the role of E-cadherin in the selective permeation of hypericin in bladder cancer tissues. Clinical studies were done on a series of 43 histologically graded bladder cancer biopsy specimens, obtained from 28 patients who received intravesical instillations with 8μM hypericin solution for at least 2 hours. Immunohistochemical staining was used to assess the expression of E-cadherin, in the cryosectioned tissues. Hypericin uptake was examined by fluorescence microscopy. Immunohistochemical staining showed a clear expression of E-cadherin along the urothelial lining of the normal and pre-malignant tissues. Partial expression of these cell adhesion molecules were still observed in malignant tissues, however there was a loss of expression to variable extends along the urothelium. Thus, loss of intercellular adhesion can be associated with enhanced hypericin permeation through paracellular diffusion.

Paper Details

Date Published: 13 July 2009
PDF: 9 pages
Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 73806C (13 July 2009); doi: 10.1117/12.828461
Show Author Affiliations
S. Sasidharan Lucky, National Cancer Ctr. Singapore (Singapore)
Ramaswamy Bhuvaneswari, National Cancer Ctr. Singapore (Singapore)
William W. L. Chin, National Cancer Ctr. Singapore (Singapore)
Weber K. O. Lau, Singapore General Hospital (Singapore)
Malini C. D. Olivo, National Cancer Ctr. Singapore (Singapore)
National Univ. of Singapore (Singapore)
Singapore Bioimaging Consortium (Singapore)


Published in SPIE Proceedings Vol. 7380:
Photodynamic Therapy: Back to the Future

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