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Proceedings Paper

Luminescent dye-doped or rare-earth-doped monodisperse silica nanospheres as efficient labels in DNA microarrays
Author(s): F. Enrichi; R. Riccò; A. Meneghello; R. Pierobon; F. Marinello; P. Schiavuta
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Paper Abstract

Luminescent nanoparticles are gaining more and more interest in bio-labeling and bio-imaging applications, like for example DNA microarray. This is a high-throughput technology used for detection and quantification of nucleic acid molecules and other ones of biological interest. The analysis is resulting by specific hybridization between probe sequences deposited in array and a target ss-DNA usually expressed by PCR and functionalized by a fluorescent dye. These organic labels have well known disadvantages like photobleaching and limited sensitivity. Quantum dots may be used as alternatives, but they present troubles like blinking, toxicity and excitation wavelengths out of the usual range of commercial instruments, lowering their efficiency. Therefore in this work we investigate a different strategy, based on the use of inorganic silica nanospheres incorporating standard luminescent dyes or rare earth doped nanocrystals. In the first case it is possible to obtain a high luminescence emission signal, due to the high number of dye molecules that can be accommodated into each nanoparticle, reduced photobleaching and environmental protection of the dye molecules thanks to the encapsulation in the silica matrix. In the second case, rare earths exhibit narrow emission bands (easy identification), large Stokes shifts (efficient discrimination of excitation and emission) and long luminescence lifetimes (possibility to perform time-delayed analysis) which can be efficiently used for the improvement of signal to noise ratio. The synthesis and characterization of good luminescent silica spheres either by organic dye-doping or by rare-earth-doping are investigated and reported. Moreover, their application in the DNA microarray technology in comparison to the use of standard molecular fluorophores or commercial quantum dots is discussed. The cheap and easy synthesis of these luminescent particles, the stability in water, the surface functionalization and bio-compatibility makes them very promising for present and future applications in bio-labeling and bio-imaging.

Paper Details

Date Published: 1 September 2009
PDF: 10 pages
Proc. SPIE 7393, Nanophotonic Materials VI, 73930M (1 September 2009); doi: 10.1117/12.828217
Show Author Affiliations
F. Enrichi, CIVEN (Italy)
R. Riccò, CIVEN (Italy)
A. Meneghello, CIVEN (Italy)
R. Pierobon, CIVEN (Italy)
F. Marinello, CIVEN (Italy)
P. Schiavuta, CIVEN (Italy)

Published in SPIE Proceedings Vol. 7393:
Nanophotonic Materials VI
Stefano Cabrini; Taleb Mokari, Editor(s)

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