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Proceedings Paper

Single molecule observations of DNA hybridization kinetics
Author(s): Ana Jofre; Jason Case; Sean Hicks
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Paper Abstract

Two 25 base-pair complementary DNA strands are encapsulated within an optically trapped nano-droplet, and we observe the kinetics of their hybridization in dynamic equilibrium via single molecule fluorescence resonance energy transfer (FRET) as a function of temperature and of the solution's NaCl concentration. We have observed binding and unbinding events between the two freely diffusing DNA strands, and our measurements reveal that the duplex can exist in multiple conformational states at elevated temperatures and low concentrations of NaCl.

Paper Details

Date Published: 20 August 2009
PDF: 10 pages
Proc. SPIE 7400, Optical Trapping and Optical Micromanipulation VI, 740014 (20 August 2009); doi: 10.1117/12.828027
Show Author Affiliations
Ana Jofre, The Univ. of North Carolina at Charlotte (United States)
Jason Case, The Univ. of North Carolina at Charlotte (United States)
Sean Hicks, The Univ. of North Carolina at Charlotte (United States)


Published in SPIE Proceedings Vol. 7400:
Optical Trapping and Optical Micromanipulation VI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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