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Proceedings Paper

Microfluidic system based on the digital holography microscope for analysis of motile sperm
Author(s): G. Di Caprio; G. Coppola; S. Grilli; P. Ferraro; R. Puglisi; D. Balduzzi; A. Galli
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Paper Abstract

Digital holography (DH) has been employed in the retrieval of three dimensional images of bull's sperm heads. The system allows a three dimensional analysis of the sperm morphology by means of a Digital Holographic Microscope (DHM). Microscopic holography measurements are performed by projecting a magnified image of a microscopic hologram plane onto a CCD plane. This could constitute the basis of an alternative method for the zoothecnic industry aimed at the investigation of morphological features and the sorting of the motile sperm cells. Indeed, one of the main advantages of digital holography consists in its full non-invasivity and in the capability of investigating the shape of the sperm cells without altering their characteristics. In particular the proposed technique could be applied to investigate the frequency of aberrant spermatozoa. Until now, in fact, such industrial investigations have been mainly performed by means of specific painting probes: unfortunately this technique dramatically reduces the vitality of the sperm cells and can even cause chromosome aberration, making them useless for the zootechnical applications.

Paper Details

Date Published: 17 June 2009
PDF: 9 pages
Proc. SPIE 7389, Optical Measurement Systems for Industrial Inspection VI, 738907 (17 June 2009); doi: 10.1117/12.827826
Show Author Affiliations
G. Di Caprio, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
Univ. degli Studi di Napoli Federico II (Italy)
G. Coppola, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
S. Grilli, Istituto Nazionale di Ottica Applicata, CNR (Italy)
P. Ferraro, Istituto Nazionale di Ottica Applicata, CNR (Italy)
R. Puglisi, Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy)
D. Balduzzi, Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy)
A. Galli, Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy)


Published in SPIE Proceedings Vol. 7389:
Optical Measurement Systems for Industrial Inspection VI
Peter H. Lehmann, Editor(s)

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