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Proceedings Paper

Reactive derivatives of gramicidin enable light- and ion-modulated ion channels
Author(s): Michael X. Macrae; Steven Blake; Thomas Mayer; Michael Mayer; Jerry Yang
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Paper Abstract

Detection of chemical processes on a single molecule scale is the ultimate goal of sensitive analytical assays. We have explored methods to detect chemical analytes in solution using synthetic derivatives of gramicidin A (gA). We exploited the functional properties of an ion channel-forming peptideg—gA—to report changes in the local environment near the opening of these semi-synthetic nanopores upon exposure to specific external stimuli. These peptide-based nanosensors detect reaction-induced changes in the chemical or physical properties of functional groups presented at the opening of the pore. This paper discusses the development of gA-based sensors for detecting external factors such as metal ions in solution or for detecting specific wavelengths of light. We propose that gA-based ion channel sensors offer tremendous potential for ultra sensitive functional detection since a single chemical modification of each individual sensing element can lead to readily detectable changes in channel conductance.

Paper Details

Date Published: 21 August 2009
PDF: 11 pages
Proc. SPIE 7397, Biosensing II, 739709 (21 August 2009); doi: 10.1117/12.827686
Show Author Affiliations
Michael X. Macrae, Univ. of California, San Diego (United States)
Steven Blake, Univ. of California, San Diego (United States)
Thomas Mayer, Univ. of Michigan (United States)
Michael Mayer, Univ. of Michigan (United States)
Jerry Yang, Univ. of California, San Diego (United States)

Published in SPIE Proceedings Vol. 7397:
Biosensing II
Manijeh Razeghi; Hooman Mohseni, Editor(s)

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