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Proceedings Paper

Laser guidance-based cell detection
Author(s): Zhen Ma; Julie X. Yun; Yangzhang Wei; Karen J. K. Burg; Xiaocong Yuan; Bruce Z. Gao
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Paper Abstract

Laser guidance is the technique that uses a weakly convergent laser beam to trap particles radially in the center of the beam and simultaneously propel them along the beam propagation axis with a travelling distance over millimeters. In this paper, we describe the applications of laser guidance to detect different cell types, including those of phenotypically transformed or gene-modified cells, especially for situations in which fluorescent markers used in flow cytometry for cell detection are not available or their application is contraindicated by clinical restriction. The optical force, which determines the guidance speed of the cell, is dependent on the characteristics, such as size, shape, composition and refractive index, of the cell being guided. Therefore, by measuring the guidance speed of the cell along the laser beam, cells with different properties can be effectively distinguished. We report two experimental results: 1) the laser-guidance system could significantly distinguish the metastatic cancer cell type 4T1 from its non-metastatic counterpart 4T07, which could not be achieved by using a high magnification microscope; 2) The laser-guidance experiment demonstrated that only one gene modification between L-10 and TC-1 cells resulted in ~40% difference in guidance speed. These experimental data indicate that laser guidance can be used to detect subtle differences between sub-cell types.

Paper Details

Date Published: 20 August 2009
PDF: 11 pages
Proc. SPIE 7400, Optical Trapping and Optical Micromanipulation VI, 74000N (20 August 2009); doi: 10.1117/12.827360
Show Author Affiliations
Zhen Ma, Clemson Univ. (United States)
Julie X. Yun, Clemson Univ. (United States)
Yangzhang Wei, Greenville Hospital System (United States)
Karen J. K. Burg, Clemson Univ. (United States)
Xiaocong Yuan, Nankai Univ. (China)
Bruce Z. Gao, Clemson Univ. (United States)


Published in SPIE Proceedings Vol. 7400:
Optical Trapping and Optical Micromanipulation VI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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