Proceedings Paper
High-resolution optical tweezers for investigating DNA-binding/translocating molecular motors| Format | Member Price | Non-Member Price |
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Paper Abstract
A double-trap optical tweezers instrument was constructed and its spatial resolution measured. The instrument features
real-time control that allows feedback based position- and force-clamping experiments. To study RNA-polymerization
by QDE-1, an RNA-dependent RNA-polymerase, we tethered a 7250 nt single-stranded DNA molecule between two
optically trapped microspheres. Preliminary constant-force extension trajectories and force-extension curves were
collected.
Paper Details
Date Published: 20 August 2009
PDF: 8 pages
Proc. SPIE 7400, Optical Trapping and Optical Micromanipulation VI, 740007 (20 August 2009); doi: 10.1117/12.826470
Published in SPIE Proceedings Vol. 7400:
Optical Trapping and Optical Micromanipulation VI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)
PDF: 8 pages
Proc. SPIE 7400, Optical Trapping and Optical Micromanipulation VI, 740007 (20 August 2009); doi: 10.1117/12.826470
Show Author Affiliations
Anders E. Wallin, Univ. of Helsinki (Finland)
Heikki Ojala, Univ. of Helsinki (Finland)
Gabija Ziedaite, Univ. of Helsinki (Finland)
Heikki Ojala, Univ. of Helsinki (Finland)
Gabija Ziedaite, Univ. of Helsinki (Finland)
Linda Degerth, Univ. of Helsinki (Finland)
Dennis Bamford, Univ. of Helsinki (Finland)
Edward Haeggström, Univ. of Helsinki (Finland)
Dennis Bamford, Univ. of Helsinki (Finland)
Edward Haeggström, Univ. of Helsinki (Finland)
Published in SPIE Proceedings Vol. 7400:
Optical Trapping and Optical Micromanipulation VI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)
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