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Proceedings Paper

High-resolution optical tweezers for investigating DNA-binding/translocating molecular motors
Author(s): Anders E. Wallin; Heikki Ojala; Gabija Ziedaite; Linda Degerth; Dennis Bamford; Edward Haeggström
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Paper Abstract

A double-trap optical tweezers instrument was constructed and its spatial resolution measured. The instrument features real-time control that allows feedback based position- and force-clamping experiments. To study RNA-polymerization by QDE-1, an RNA-dependent RNA-polymerase, we tethered a 7250 nt single-stranded DNA molecule between two optically trapped microspheres. Preliminary constant-force extension trajectories and force-extension curves were collected.

Paper Details

Date Published: 20 August 2009
PDF: 8 pages
Proc. SPIE 7400, Optical Trapping and Optical Micromanipulation VI, 740007 (20 August 2009); doi: 10.1117/12.826470
Show Author Affiliations
Anders E. Wallin, Univ. of Helsinki (Finland)
Heikki Ojala, Univ. of Helsinki (Finland)
Gabija Ziedaite, Univ. of Helsinki (Finland)
Linda Degerth, Univ. of Helsinki (Finland)
Dennis Bamford, Univ. of Helsinki (Finland)
Edward Haeggström, Univ. of Helsinki (Finland)

Published in SPIE Proceedings Vol. 7400:
Optical Trapping and Optical Micromanipulation VI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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