Share Email Print
cover

Proceedings Paper

Evidence of chemotaxis by quantitative measurement of the force vectors of Trypanossoma cruzi in the vicinity of the Rhodnius prolixus midgut wall cell
Author(s): A. A. de Thomaz; D. B. Almeida; A. Fontes; C. V. Stahl; J. R. Santos-Mallet; S. A. O. Gomes; D. Feder; C. L. Cesar
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

In this work we used a methodology to study chemotaxis of Trypanossoma cruzi (T. Cruzi) in real time using an Optical Tweezers system. Trapped beads were used as a force transducer for measuring forces of the same order of magnitude as typical forces induced by flagellar motion. Optical Tweezers allowed real time measurements of the force vectors, strength and direction, of living parasites under chemical or other kinds of gradients. This seems to be the ideal tool to perform observations of taxis response of cells and microorganisms with high sensitivity to capture instantaneous responses to a given stimulus. We applied this methodology to investigate the T. cruzi under distinct situations: the parasite alone and in the presence of its insect-vector Rhodnius prolixus (R. prolixus).

Paper Details

Date Published: 20 August 2009
PDF: 6 pages
Proc. SPIE 7400, Optical Trapping and Optical Micromanipulation VI, 740009 (20 August 2009); doi: 10.1117/12.826314
Show Author Affiliations
A. A. de Thomaz, UNICAMP (Brazil)
D. B. Almeida, UNICAMP (Brazil)
A. Fontes, Univ. Federal de Pernambuco (Brazil)
C. V. Stahl, IOC-FIOCRUZ (Brazil)
J. R. Santos-Mallet, IOC-FIOCRUZ (Brazil)
S. A. O. Gomes, IOC-FIOCRUZ (Brazil)
D. Feder, Univ. Federal Fluminense (Brazil)
C. L. Cesar, UNICAMP (Brazil)


Published in SPIE Proceedings Vol. 7400:
Optical Trapping and Optical Micromanipulation VI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

© SPIE. Terms of Use
Back to Top