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Proceedings Paper

Exploiting plasmonics in biosensing and bioimaging: monitoring cell receptors with surface enhanced spectroscopy and microscopy
Author(s): Li-Lin Tay; David Kennedy; John Hulse; John P. Pezaki; Jeff Fraser; Xiaohua Wu
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Paper Abstract

The plasmon resonance of noble metal nanoparticles (NP) manifests itself in a variety of extraordinary optical properties. Resonant excitation of the conduction electrons by incident radiation generates a localized surface plasmon resonance (LSPR) that is responsible for a variety of surface enhanced optical phenomena. This unique optical property coupled with well-established surface chemistry allows us to utilize both Ag and Au NP as optical contrasting agents to probe and monitor the surface receptors of cells. We have employed two plasmon-assisted optical techniques (namely, surface enhanced Raman scattering, and resonant Rayleigh scattering) to monitor the adrenergic receptors in mammalian cardiomyocyte cells that have been labeled with functionalized Ag NPs. In this study, a unique Raman reporter molecule, 4-(mercaptomethyl)benzonitrile, was developed to provide an easily identifiable vibration, the C≡N stretch, in a spectral window free from Raman bands of cell constituents and other biomolecules used in receptor crosslinking and surface passivation. Successfully labeled cells were then monitored with both optical techniques. Both techniques are related through the plasmonic properties of the noble metal NP and combined with high resolution imaging techniques; we outline the importance that different NP architectures play in the different imaging techniques. Furthermore, we will discuss the instrumentation and plasmonic implications in the design of NP best suited for such multimodal imaging approaches.

Paper Details

Date Published: 2 September 2009
PDF: 9 pages
Proc. SPIE 7395, Plasmonics: Nanoimaging, Nanofabrication, and their Applications V, 73950O (2 September 2009); doi: 10.1117/12.825358
Show Author Affiliations
Li-Lin Tay, National Research Council Canada (Canada)
David Kennedy, National Research Council Canada (Canada)
John Hulse, National Research Council Canada (Canada)
John P. Pezaki, National Research Council Canada (Canada)
Jeff Fraser, National Research Council Canada (Canada)
Xiaohua Wu, National Research Council Canada (Canada)

Published in SPIE Proceedings Vol. 7395:
Plasmonics: Nanoimaging, Nanofabrication, and their Applications V
Satoshi Kawata; Vladimir M. Shalaev; Din Ping Tsai, Editor(s)

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