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Proceedings Paper

Hyperbranched polysiloxysilane nanoparticles for nonviral gene delivery vectors and nanoprobes
Author(s): Won Jin Kim; Adela C. Bonoiu; Kwang-Sup Lee; Teruaki Hayakawa; Cheng Xia; Masa-aki Kakimoto; Haridas E. Pudavar; Paras N. Prasad
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Paper Abstract

We report an approach to produce predefined surface charge tunable gene delivery vectors using siloxysilsane-based polymer for gene delivery studies. To obtain nonviral vectors, new series of hyperbranched polysiloxysilane (HBPS) were synthesized, and the end groups in polymer structures have modified with hydrophilic molecules; in other words, carboxylic acid and quaternary ammonium groups were employed into terminal structures to give the amphiphilicity. The novelty of these amphiphilic HBPS polymers lies in the fact that nanoparticles with different zeta potential (surface charge density) can be easily tailored and functionalized. These polymeric nanoparticles which containing various chemical groups on the surface indicated altered surface charge distributions (from -40 to +64mV). Finally, the use of these nanoparticles as efficient gene delivery vectors was demonstrated by means of in vitro transfection study using β- galactosidase plasmid and pEGFP-N1 plasmid, and the most efficient combination was obtained using HBPS-CN30:70.

Paper Details

Date Published: 20 August 2009
PDF: 6 pages
Proc. SPIE 7403, Nanobiosystems: Processing, Characterization, and Applications II, 74030H (20 August 2009); doi: 10.1117/12.824518
Show Author Affiliations
Won Jin Kim, Hannam Univ. (Korea, Republic of)
Univ. at Buffalo (United States)
Adela C. Bonoiu, Univ. at Buffalo (United States)
Kwang-Sup Lee, Hannam Univ. (Korea, Republic of)
Teruaki Hayakawa, Tokyo Institute of Technology (Japan)
Cheng Xia, Tokyo Institute of Technology (Japan)
Masa-aki Kakimoto, Tokyo Institute of Technology (Japan)
Haridas E. Pudavar, Univ. at Buffalo (United States)
Paras N. Prasad, Univ. at Buffalo (United States)

Published in SPIE Proceedings Vol. 7403:
Nanobiosystems: Processing, Characterization, and Applications II
Norihisa Kobayashi; Fahima Ouchen; Ileana Rau, Editor(s)

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