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Proceedings Paper

Nano-engineering of materials for nonlinear optical imaging
Author(s): Koen Clays; Jos Vanderleyden; Harry L Anderson
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Paper Abstract

We discuss the use of genetically modified fluorescent proteins (FPs) and a specially engineered chromophore for nonlinear optical imaging. While it is clear that FPs can be used for two-photon fluorescence (TPF) microscopy, we show that they also exhibit a large second-order nonlinear optical response, useful for second-harmonic generation (SHG) imaging. The relation between the linear and nonlinear optical properties in a small series of FPs will be discussed. We also present a new optimized chromophore for combined TPF and SHG microscopy and we show imaging results obtained on this chromophore on a water droplet model system.

Paper Details

Date Published: 20 August 2009
PDF: 9 pages
Proc. SPIE 7413, Linear and Nonlinear Optics of Organic Materials IX, 74130J (20 August 2009); doi: 10.1117/12.824517
Show Author Affiliations
Koen Clays, Katholieke Univ. Leuven (Belgium)
Institute for Nanoscale Physics and Chemistry (Belgium)
Jos Vanderleyden, Katholieke Univ. Leuven (Belgium)
Institute for Nanoscale Physics and Chemistry (Belgium)
Harry L Anderson, Univ. of Oxford (United Kingdom)


Published in SPIE Proceedings Vol. 7413:
Linear and Nonlinear Optics of Organic Materials IX
Theodore G. Goodson III, Editor(s)

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