Share Email Print
cover

Proceedings Paper

Five-color fluorescent imaging in living tumor cells
Author(s): Liang Wang; Jie Yang; Jun Chu; Qingming Luo; Zhihong Zhang
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The fluorescent probes based on fluorescent proteins (FP) have been widely used to investigate the molecules of interest in living cells. It is well-known that the molecular events in the living cells are very complicate and all of the cell activities are involved by multi-molecular interaction. With the development of novel fluorescent protein mutants and imaging technology, the molecular signal in living cells could be detected accurately. In this study, with the appropriate targeting signals, the fluorescent proteins were localized to plasma membrane (Rac1-mCerulean), Golgi membrane (EYFP-go), ER membrane (RFP2-er), mitochondrial membrane (RFP1-mt). Cultured Hela cells were cotransfected with these four plasmids, and 36 h later, labeled with Hoechst33258 which located in the nucleus of a living cell. Using a confocal microscopy, with 405 nm, 458 nm and 514 nm laser lines employed respectively, a five-color fluorescent image was obtained in which five subcellular structures were clearly shown in living cells. The technique of multi-color imaging in a single cell provides a powerful tool to simultaneously study the multi-molecular events in living cells.

Paper Details

Date Published: 6 March 2009
PDF: 5 pages
Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72801Z (6 March 2009); doi: 10.1117/12.824017
Show Author Affiliations
Liang Wang, Huazhong Univ. of Science and Technology (China)
Jie Yang, Huazhong Univ. of Science and Technology (China)
Jun Chu, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)
Zhihong Zhang, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 7280:
Seventh International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

© SPIE. Terms of Use
Back to Top