Share Email Print
cover

Proceedings Paper

Study on the SPR responses of various DNA probe concentrations by parallel scan spectral SPR imaging
Author(s): Suihua Ma; Le Liu; Weiping Lu; Yaou Zhang; Yonghong He; Jihua Guo
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

SPR sensors have become a high sensitive and label free method for characterizing and quantifying chemical and biochemical interactions. However, the relations between the SPR refractive index response and the property (such as concentrations) of biochemical probes are still lacking. In this paper, an experimental study on the SPR responses of varies concentrations of Legionella pneumophila mip DNA probes is presented. We developed a novel two-dimensional SPR sensing technique-parallel scan spectral SPR imaging-to detect an array of mip gene probes. This technique offers quantitative refractive index information with a high sensing throughput. By detecting mip DNA probes with different concentrations, we obtained the relations between the SPR refractive index response and the concentrations of mip DNA probes. These results are valuable for design and developing SPR based mip gene biochips.

Paper Details

Date Published: 6 March 2009
PDF: 5 pages
Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72801H (6 March 2009); doi: 10.1117/12.823908
Show Author Affiliations
Suihua Ma, Tsinghua Univ. (China)
Le Liu, Tsinghua Univ. (China)
Weiping Lu, Third Military Medical Univ. (China)
Yaou Zhang, Tsinghua Univ. (China)
Yonghong He, Tsinghua Univ. (China)
Jihua Guo, Tsinghua Univ. (China)


Published in SPIE Proceedings Vol. 7280:
Seventh International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

© SPIE. Terms of Use
Back to Top