Share Email Print
cover

Proceedings Paper

Brca1/p53 deficient mouse breast tumor hemodynamics during hyperoxic respiratory challenge monitored by a novel wide-field functional imaging (WiFI) system
Author(s): Austin Moy; Jae G. Kim; Eva Y. H. P. Lee; Bruce Tromberg; Albert Cerussi; Bernard Choi
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Current imaging modalities allow precise visualization of tumors but do not enable quantitative characterization of the tumor metabolic state. Such quantitative information would enhance our understanding of tumor progression and response to treatment, and to our overall understanding of tumor biology. To address this problem, we have developed a wide-field functional imaging (WiFI) instrument which combines two optical imaging modalities, spatially modulated imaging (MI) and laser speckle imaging (LSI). Our current WiFI imaging protocol consists of multispectral imaging in the near infrared (650-980 nm) spectrum, over a wide (7 cm × 5 cm) field of view. Using MI, the spatially-resolved reflectance of sinusoidal patterns projected onto the tissue is assessed, and optical properties of the tissue are estimated using a Monte Carlo model. From the spatial maps of local absorption and reduced scattering coefficients, tissue composition information is extracted in the form of oxy-, deoxy-, and total hemoglobin concentrations, and percentage of lipid and water. Using LSI, the reflectance of a 785 nm laser speckle pattern on the tissue is acquired and analyzed to compute maps of blood perfusion in the tissue. Tissue metabolism state is estimated from the values of blood perfusion, volume and oxygenation state. We currently are employing the WiFI instrument to study tumor development in a BRCA1/p53 deficient mice breast tumor model. The animals are monitored with WiFI during hyperoxic respiratory challenge. At present, four tumors have been measured with WiFI, and preliminary data suggest that tumor metabolic changes during hyperoxic respiratory challenge can be determined.

Paper Details

Date Published: 23 February 2009
PDF: 5 pages
Proc. SPIE 7174, Optical Tomography and Spectroscopy of Tissue VIII, 71742L (23 February 2009); doi: 10.1117/12.823745
Show Author Affiliations
Austin Moy, Beckman Laser Institute and Medical Clinic (United States)
Univ. of California, Irvine (United States)
Jae G. Kim, Beckman Laser Institute and Medical Clinic (United States)
Eva Y. H. P. Lee, Univ. of California, Irvine (United States)
Bruce Tromberg, Beckman Laser Institute and Medical Clinic (United States)
Univ. of California, Irvine (United States)
Albert Cerussi, Beckman Laser Institute and Medical Clinic (United States)
Bernard Choi, Beckman Laser Institute and Medical Clinic (United States)
Univ. of California, Irvine (United States)


Published in SPIE Proceedings Vol. 7174:
Optical Tomography and Spectroscopy of Tissue VIII
Bruce J. Tromberg; Arjun G. Yodh; Mamoru Tamura; Eva M. Sevick-Muraca; Robert R. Alfano, Editor(s)

© SPIE. Terms of Use
Back to Top